Student Work
Investigation of the Propensity for Self-Association of the N-Terminal Domain of Annexin A2 in the Presence of Anionic Lipids
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open in viewerAnnexin A2 (ANXA2), a calcium-dependent membrane binding protein shown to promote membrane domain formation, is implicated in many cellular processes. In order to determine if ANXA2 promotes domain formation by self-associating with the N-terminal domain of adjacent ANXA2 proteins upon binding anionic phospholipids, Forster Resonance Energy Transfer (FRET) assays were performed using ANXA2 proteins fluorescently labeled at the singular, exposed N-terminal cysteine residue. No FRET transfer was observed, suggesting that under these experimental conditions the N-terminal domains do not interact.
- This report represents the work of one or more WPI undergraduate students submitted to the faculty as evidence of completion of a degree requirement. WPI routinely publishes these reports on its website without editorial or peer review.
- Creator
- Publisher
- Identifier
- E-project-042417-210748
- Advisor
- Year
- 2017
- Date created
- 2017-04-24
- Resource type
- Major
- Rights statement
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Thumbnail | Title | Visibility | Embargo Release Date | Actions |
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CORNWELL_4242017_Final_MQP_Draft2.pdf | Public | Download | ||
MQP_POSTER_CORNWELL_4242017.pdf | Public | Download |
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