Compounds that stimulate interferon production in cells might have therapeutic effects against viruses that prevent the activation of interferon production. Our lab previously identified a compound as potential up-regulators of interferon production.. This project confirmed the effects of the compound and then analyzed the mechanism of interferon production using RT-PCR, bioassays, nanostring assays, and residual viral levels in vitro to determine the ability of the compounds to protect against viral infection. The results show that interferon production can be stimulated in a variety of cell lines (HEK293 cells, macrophages, dendritic cells, and fibroblast cells) and the protein RIG-I is necessary for interferon production by the compound.

• This report represents the work of one or more WPI undergraduate students submitted to the faculty as evidence of completion of a degree requirement. WPI routinely publishes these reports on its website without editorial or peer review.

Creator

• Krawic, Casey Anne
• Crouse, Jacquelin Amber-Blair

Contributors

• Finberg, Robert

Publisher

• Worcester Polytechnic Institute

Identifier

• E-project-032212-091007

Advisor

• Adams, David S.

Year

• 2012

Sponsor

• Umass Medical Center

Date created

• 2012-03-22

Resource type

• Major Qualifying Project

Major

• Biology & Biotechnology

Rights statement

• In Copyright

Last modified

• 2023-10-09