Early in C. elegans development, several neurons must migrate to specific locations within the body. Mutations in mig-10 result in incomplete migration of several neurons, as well as defects in egg laying and excretory cell development. We tested the ability of each of the two mig-10 splice forms to rescue the egg laying defect; partial rescue was observed. These results indicate that perhaps more than one isoform of the MIG-10 protein must be expressed simultaneously in multiple cells in order to achieve total mutant rescue. To test the hypothesis that expression in specific neurons is required for rescue of the migration and excretory cell defects, constructs were made to express each mig-10 splice form in a particular neuron.

• This report represents the work of one or more WPI undergraduate students submitted to the faculty as evidence of completion of a degree requirement. WPI routinely publishes these reports on its website without editorial or peer review.

Creator

• Santos, Ashley Renee
• Ziolek, Agnieszka B.
• Epstein, Sarah R.

Publisher

• Worcester Polytechnic Institute

Identifier

• E-project-042706-145413

Advisor

• Ryder, Elizabeth F.

Year

• 2006

Date created

• 2006-04-27

Resource type

• Major Qualifying Project

Major

• Biology & Biotechnology

Rights statement

• In Copyright

Last modified

• 2021-02-02