Glucose Regulated Protein-78 (GRP78) is an ER stress chaperone whose decreased expression is associated with Type II diabetes. The goal of this project was to create a knockout mouse for GRP78 in adipocytes to allow future studies on the effects of GRP78 absence in that tissue. Mice hemizygous for Cre-recombinase under the control of the adipocyte-specific and tamoxifen-inducible promoter adiponectin (Ad-Cre) were bred with mice heterozygous for floxed GRP78 to produce a subset of pups of the desired genotype (homozygous floxed GRP78, hemizygous for Ad-Cre). Pups were screened by PCR genotyping tail snip DNA for Ad-Cre and GRP78. Two of the F1 male pups were of the exact desired genotype.

• This report represents the work of one or more WPI undergraduate students submitted to the faculty as evidence of completion of a degree requirement. WPI routinely publishes these reports on its website without editorial or peer review.

Creator

• Baara, Farrah Munther
• Ali, Noor Mohammad

Publisher

• Worcester Polytechnic Institute

Identifier

• E-project-042513-114932

Advisor

• Adams, David S.

Year

• 2013

Date created

• 2013-04-25

Resource type

• Major Qualifying Project

Major

• Biochemistry
• Biology & Biotechnology

Rights statement

• In Copyright