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FLUORESENCE MICROSCOPY OF NLR INFLAMMASOME INTERACTIONS

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Inflammation is initiated by the innate immune system to remove harmful stimuli and heal infections. Multiprotein complexes, called inflammasomes, process inflammatory cytokines such as interleukin (IL)-1 beta and IL-18 and are essential for optimal host response. Infammasomes are typically comprised of one or more nod-like receptor (NLR), a pro-caspase protease, and an adaptor molecule. NLRP3 and NLRP12 are NLRs with specificities towards certain pathogens and vaccines; however, aspects of their activation remain unclear. To investigate a possible mode of assembly, we generated fluorescently tagged NLR pyrin domain-containing protein (NLRP) plasmids for transformation into cells. Expression of NLRP3 and NLRP12 inflammasome components was successfully observed by fluorescence microscopy.

  • This report represents the work of one or more WPI undergraduate students submitted to the faculty as evidence of completion of a degree requirement. WPI routinely publishes these reports on its website without editorial or peer review.
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  • E-project-110612-115451
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  • 2012
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  • 2012-11-06
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