Mesenchymal stem cells (MSCs) are multipotent stromal cells that have the potential to differentiate into various cells lineages, including adipocytes, chondrocytes, osteocytes, and fibroblasts. Due to their ability to regenerate damaged tissues, MSCs have the potential to be the future of new cell-based therapies. However, identification of MSCs has not been standardized. The purpose of this project was to identify parameters for isolating MSCs from murine bone marrow. This was accomplished by combining a staining protocol that utilizes the unique surface characteristics of MSCs with the negative selection for CD45 and Ter-119, and then the negative selection for CD44 in the remaining cell population.

• This report represents the work of one or more WPI undergraduate students submitted to the faculty as evidence of completion of a degree requirement. WPI routinely publishes these reports on its website without editorial or peer review.

Creator

• Parker, Heather Roark

Contributors

• Gerstein, Rachel

Publisher

• Worcester Polytechnic Institute

Identifier

• E-project-042413-225140

Advisor

• Adams, David S.

Year

• 2013

Sponsor

• UMass Medical School

Date created

• 2013-04-24

Resource type

• Major Qualifying Project

Major

• Biology & Biotechnology

Rights statement

• In Copyright

Last modified

• 2023-10-09