Student Work

Root and Shoot Elicitation in Artemisia annua: Chitosan and Salicylic Acid Effect on Artemisinin and Flavonoid Biosynthesis

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Our project analyzed root and shoot elicitation of the anti-malarial drug-producing plant, Artemisia annua, using the elicitors chitosan and salicylic acid. We accomplished this by applying chitosan and salicylic acid to the roots and shoots of hydroponically grown A. annua and harvesting both the tips of the apical meristem and the third through fifth leaves from the tips. The antimalarial drug, artemisinin, and flavonoids that act synergistically with the drug were extracted and quantified using GC/MS and an aluminum chloride assay, respectively. We observed higher concentrations of artemisinin in the tips of plants treated with chitosan via the roots indicating chitosan elicits artemisinin biosynthesis and that the roots play an important role in the production of artemisinin.

  • This report represents the work of one or more WPI undergraduate students submitted to the faculty as evidence of completion of a degree requirement. WPI routinely publishes these reports on its website without editorial or peer review.
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  • E-project-042313-200714
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  • 2013
Date created
  • 2013-04-23
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Permanent link to this page: https://digital.wpi.edu/show/9c67wp318