Design of a plasmid expression system for simultaneously co-expressing high levels of multiple globin subunits

Kizner, Matthew T

Student Work

Design of a plasmid expression system for simultaneously co-expressing high levels of multiple globin subunits

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The heterotetrameric hemoglobin (HbII) from the blood clam Scapharca inequivalvis assembles from two heterodimers and provides a simple system to analyze the allosteric mechanisms in which unlike subunits bind ligands cooperatively. By finding a more effective way to produce HbII in lab, as well as HbI, the homodimeric hemoglobin from the same organism, work can continue forward on the allosteric and kinetic studies of this interesting protein.

This report represents the work of one or more WPI undergraduate students submitted to the faculty as evidence of completion of a degree requirement. WPI routinely publishes these reports on its website without editorial or peer review.

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