This MQP compared the effects that varying levels of 5-azacytidine had on growth and artemisinin levels in transformed hairy roots of Artemisia annua. Also compared was the difference in DNA methylation levels between shoots, roots and transformed roots. The intent of this project was to test the effects DNA methylation may play in slowing artemisinin production in older hairy root cultures of Artemisia annua by attempting to demethylate the DNA of older root cultures through the incorporation of 5-azacytidine. Although the results showed that 5-azacytidine is an inhibitor of growth in hairy roots of Artemisia annua, there was no apparent increase in artemisinin.

• This report represents the work of one or more WPI undergraduate students submitted to the faculty as evidence of completion of a degree requirement. WPI routinely publishes these reports on its website without editorial or peer review.

Creator

• Fuller, Jason Henry

Contributors

• Wobbe, Kristin K.

Publisher

• Worcester Polytechnic Institute

Identifier

• E-project-011906-170725

Advisor

• Weathers, Pamela

Year

• 2006

Date created

• 2006-01-19

Resource type

• Major Qualifying Project

Major

• Biology & Biotechnology

Rights statement

• In Copyright

Last modified

• 2023-12-05