Typically, new neurons migrate randomly out of the RMS into the olfactory bulb. This experiment seeks to view the effect of the release of GABA from active granule cells, which is hypothesized to cause directed migration of neurons in the olfactory bulb. Mouse granule cells were experimentally altered through use of lentiviral vectors delivering genes for ion channel Nachbac. Then, fluorescence microscopy, and the Neurolucida computer module were used to investigate the behavior and distribution of new neurons in the olfactory bulb. Data analysis and experiments in the UMASS Lab show that experimental group TRPA1 works to silence granule cell activity. It is shown that TRPA1-treated GCs work to repel new neurons from the area.

• This report represents the work of one or more WPI undergraduate students submitted to the faculty as evidence of completion of a degree requirement. WPI routinely publishes these reports on its website without editorial or peer review.

Creator

• Glavin, Shane Michael

Publisher

• Worcester Polytechnic Institute

Identifier

• E-project-042513-153041

Advisor

• Adams, David S.

Year

• 2013

Sponsor

• Carlos Lois, UMass Medical School

Date created

• 2013-04-25

Resource type

• Major Qualifying Project

Major

• Biology & Biotechnology

Rights statement

• In Copyright