Student Work
Cloning and characterization of two putative glucuronolactonases from Arabidopsis thaliana involved in ascorbate degradation
PublicDownloadable Content
open in viewerVitamin C (ascorbic acid, AsA) is the most abundant water-soluble antioxidant in plants and animals. Two plant mutants were discovered which contained high foliar AsA levels compared to controls.160 We hypothesized that these two genes are involved in AsA degradation and have performed further studies to confirm their participation in AsA metabolism.160 This work focused on cloning of ORFs, making constructs for expression in bacteria and in planta, and transformation of wild type and Arabidopsis homozygous knockouts with appropriate constructs.
- This report represents the work of one or more WPI undergraduate students submitted to the faculty as evidence of completion of a degree requirement. WPI routinely publishes these reports on its website without editorial or peer review.
- Creator
- Publisher
- Identifier
- E-project-102207-094103
- Advisor
- Year
- 2007
- Sponsor
- Date created
- 2007-10-22
- Resource type
- Major
- Rights statement
Relations
- In Collection:
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MelindaMQP_Final_Project_10.16.07.pdf | Public | Download |
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