While Phospholipase-CB1 (PLCB1) plays an important role on the plasma membrane by generating calcium signals, it also has a significant population in the cytosol where it shows affinity for Component 3 Promoter of RISC (C3PO), which is an enhancer of RNA silencing. This project focused on PLCB1s inhibition of RNA silencing in vitro and in PC12 cells by evaluating the hydrolysis of fluorescent oligonucleotides. We find that PLCB1 binds and inhibits C3PO, allowing the expression of genes whose sequence would otherwise be degraded.

• This report represents the work of one or more WPI undergraduate students submitted to the faculty as evidence of completion of a degree requirement. WPI routinely publishes these reports on its website without editorial or peer review.

Creator

• Carley, Samuel James

Publisher

• Worcester Polytechnic Institute

Identifier

• E-project-042618-133623

Advisor

• Adams, David S.
• Scarlata, Suzanne Frances

Year

• 2018

Date created

• 2018-04-26

Resource type

• Major Qualifying Project

Major

• Interdisciplinary

Rights statement

• In Copyright