Biomarker discovery and proteomics are important for disease diagnosis and monitoring. This project evaluated the effect of protein digestion conditions and sample contamination on biomarker quantitation in biological mixtures cerebrospinal fluid (CSF) and plasma. Tandem mass spectrometry was used to analyze and identify specific marker peptides. Based on the data collected, the optimal method for peptide quantitation was dependent on the matrix tested, and varied between unbiased and targeted approaches. Blood contamination in CSF was shown to not be a major concern since only one of 56 targeted peptides was slighted affected.

• This report represents the work of one or more WPI undergraduate students submitted to the faculty as evidence of completion of a degree requirement. WPI routinely publishes these reports on its website without editorial or peer review.

Creator

• Turton, Emma-Jane

Contributors

• Kate, Zhang

Publisher

• Worcester Polytechnic Institute

Identifier

• E-project-042417-162200

Advisor

• Adams, David S.

Year

• 2017

Sponsor

• Sanofi Genzyme

Date created

• 2017-04-24

Location

• Westborough

Resource type

• Major Qualifying Project

Major

• Biology & Biotechnology

Rights statement

• In Copyright