Porcine Circovirus 1 Viral Protein 3 (PCV1-VP3) selectively induces apoptosis in cancer cells. Localization within the cell is suspected to be a key factor in PCV1-VP3 inducing apoptosis. The purpose of this research is to analyze the subcellular localization of PCV1-VP3. Multiple mutants of the protein of interest were transfected into human non-small lung carcinoma cells (H1299), and transfected cells were analyzed using fluorescence microscopy and differential centrifugation. Results indicate that PCV1-VP3 associates with a perinuclear or nuclear structure. Comparison of wild type data to mutants supports the hypothesis that the nuclear export sequence (located a.a. 134-149) is significant in PCV1-VP3 localization.

• This report represents the work of one or more WPI undergraduate students submitted to the faculty as evidence of completion of a degree requirement. WPI routinely publishes these reports on its website without editorial or peer review.

Creator

• Farris, Lauren A
• Johnson, Rebekah Grace

Publisher

• Worcester Polytechnic Institute

Identifier

• E-project-042518-162550

Advisor

• Heilman, Destin

Year

• 2018

Date created

• 2018-04-25

Resource type

• Major Qualifying Project

Major

• Biochemistry

Rights statement

• In Copyright