Student Work

Examination of a putative Cu+ importer in Pseudomonas aeruginosa

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The focus of this project was to gain understanding on the putative copper importer PA5030 in the opportunistic bacteria Pseudomonas aeruginosa and its relationship to other proteins in the copper homeostasis network. First, recombination techniques were employed to successfully develop a double knockout mutant of the PA5030 gene and a different copper importer, PA1848 (ccoA). Second, in-silico exploration of the structure of the protein, accompanied by the development of a homolog filtering script, revealed two potential copper binding sites, M157-M173-H355 and C65-M185-M262, and 925 homologs that further suggest that PA5030 is a copper transporter.

  • This report represents the work of one or more WPI undergraduate students submitted to the faculty as evidence of completion of a degree requirement. WPI routinely publishes these reports on its website without editorial or peer review.
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Identifier
  • E-project-042318-010932
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Year
  • 2018
Date created
  • 2018-04-23
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Last modified
  • 2023-01-19

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Permanent link to this page: https://digital.wpi.edu/show/1r66j2561