Two bacterial samples, referred to as 14-29 & 15-6, were chosen from among several putative antibiotic-producing isolates originally discovered in a WPI laboratory course titled Microbes to Molecules: Crowdsourcing Novel Antibiotic Discovery from 2014 to present. Potential antibiotics were extracted from the samples and assayed for efficacy against E. coli and B. subtilis and characterized by HPLC. Antibiotic activity was visible to varying degrees on assay plates for each sample. Colony inhibition was demonstrated by each sample, but only 15-6 demonstrated inhibition from extraction. Identification of these antibiotics will determine their novelty and potential application for treatment of infection.

• This report represents the work of one or more WPI undergraduate students submitted to the faculty as evidence of completion of a degree requirement. WPI routinely publishes these reports on its website without editorial or peer review.

Creator

• Goodale, Liam

Publisher

• Worcester Polytechnic Institute

Identifier

• E-project-042618-110749

Advisor

• Buckholt, Michael Allan

Year

• 2018

Date created

• 2018-04-26

Location

• Worcester

Resource type

• Major Qualifying Project

Major

• Biology & Biotechnology

Rights statement

• In Copyright