Student Work

Building a T-Cell Granulysin Expression System to Investigate Tuberculosis Infection

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Tuberculosis is a bacterial infection caused by Mycobacterium tuberculosis, commonly presents as an infection of the alveolar sacs and can be difficult to treat. Granulysin, a lytic enzyme employed by CD8 cells during infection, has been shown to degrade M. tuberculosis. This project utilizes cloning, transfection, flow cytometry, and qPCR to build lentivirus and gamma-retrovirus expressing three isoforms of granulysin. These viruses can be used to infect CD8 cells and express human granulysin to better study granulysin's interaction with Mycobacterium tuberculosis. Gaining a more complete understanding of these interactions can help identify future targets to help develop a new vaccine against tuberculosis.

  • This report represents the work of one or more WPI undergraduate students submitted to the faculty as evidence of completion of a degree requirement. WPI routinely publishes these reports on its website without editorial or peer review.
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  • E-project-042419-154059
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  • 2019
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  • 2019-04-24
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