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Investigating the Role of VBH-1 in C. elegans

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VBH-1 is a DEAD-box RNA helicase found to co-immunoprecipate with CSR-1, an argonaute required for RNA-induced epigenetic activation (RNAa). Because most argonautes are thought to require an RNA helicase to function, this CO-IP data suggests that VBH-1 may be the helicase recruited by CSR-1. To test this hypothesis, experiments were conducted to assess CSR-1 function in vbh-1 mutants. These experiments found that mutations in VBH-1 do not hinder CSR-1 localization, though do result in a reduction of brood size, as well as a reduction in protein expression of CSR-1 targets. Future experiments will be carried out to assess if mutations in VBH-1 affect CSR-1 targeting.

  • This report represents the work of one or more WPI undergraduate students submitted to the faculty as evidence of completion of a degree requirement. WPI routinely publishes these reports on its website without editorial or peer review.
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  • E-project-042519-102942
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  • 2019
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  • 2019-04-25
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