Faculty Advisor

Joseph Bagshaw

Faculty Advisor

Jill Rulfs

Faculty Advisor

David Adams

Abstract

The goal of this project was to clone and express the antimicrobial peptide protegrin 1 (PG-1). Initially a yeast system was chosen but was discarded due to technical difficulties. Invitrogen's bacterial T7 expression system was chosen next to express the peptide. PG-1 expression was verified by anti-his immunoblot and then the peptide was purified by IMAC. Its activity was verified using a Bacillus subtillis radial diffusion assay.

Publisher

Worcester Polytechnic Institute

Degree Name

MS

Department

Biology & Biotechnology

Project Type

Thesis

Date Accepted

2003-04-28

Accessibility

Unrestricted

Subjects

antimicrobial peptides, protegrins, synthetic genes, Peptide antibiotics, Gene expression, Protegrins

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