Etd

Synthetic Genes for Antimicrobial Peptides

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The goal of this project was to clone and express the antimicrobial peptide protegrin 1 (PG-1). Initially a yeast system was chosen but was discarded due to technical difficulties. Invitrogen’s bacterial T7 expression system was chosen next to express the peptide. PG-1 expression was verified by anti-his immunoblot and then the peptide was purified by IMAC. Its activity was verified using a Bacillus subtillis radial diffusion assay.

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Language
  • English
Identifier
  • etd-0428103-102059
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Year
  • 2003
Date created
  • 2003-04-28
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Last modified
  • 2020-11-27

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Permanent link to this page: https://digital.wpi.edu/show/w3763681m