The goal of this project was to clone and express the antimicrobial peptide protegrin 1 (PG-1). Initially a yeast system was chosen but was discarded due to technical difficulties. Invitrogen's bacterial T7 expression system was chosen next to express the peptide. PG-1 expression was verified by anti-his immunoblot and then the peptide was purified by IMAC. Its activity was verified using a Bacillus subtillis radial diffusion assay.
Worcester Polytechnic Institute
Biology & Biotechnology
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Borrelli, Alexander P., "Synthetic Genes for Antimicrobial Peptides" (2003). Masters Theses (All Theses, All Years). 427.
antimicrobial peptides, protegrins, synthetic genes, Peptide antibiotics, Gene expression, Protegrins