Faculty Advisor

Adams, David S.

Abstract

Scientists this year used new CRISPR and TALEN technologies to efficiently edit the non-human primate genome, which may allow us to create new disease models far more accurately than current mouse models. But this new technology can place off-target mutations in the host DNA which can harm the animal. Our review of the literature and interviews with key scientists indicate that with proper genome monitoring (either by whole genome sequencing or by PCR of specific high-probability locations), careful design of guide RNAs (to avoid cross-hybridization), and screening treated embryos prior to implantation, off-target mutations can be lowered.

Publisher

Worcester Polytechnic Institute

Date Accepted

October 2014

Project Type

Interactive Qualifying Project

Accessibility

Unrestricted

Advisor Department

Biology and Biotechnology

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