Student Work

Purification of Myosin XI and Sec15 from Physcomitrella patens

Public

Downloadable Content

open in viewer

In the plant model system, the moss Physcomitrella patens, the motor protein myosin XI is essential for polarized cell growth and proliferation. Myosin XI is structurally similar to Myosin V in animals. Myosin V was found to interact, via its globular tail domain, with the exocyst subunit sec15 during secretory vesicle transport in yeast and therefore we hypothesize myosin XI and sec15 would interact in P. patens. In this study, we recombinantly expressed and purified two globular tail domain constructs from myosin XI and full length sec15 from P. patens. The purified proteins can be used in future experiments to analyze the proposed binding interaction of myosin XI and sec15.

  • This report represents the work of one or more WPI undergraduate students submitted to the faculty as evidence of completion of a degree requirement. WPI routinely publishes these reports on its website without editorial or peer review.
Creator
Publisher
Identifier
  • E-project-042513-102503
Advisor
Year
  • 2013
Sponsor
Date created
  • 2013-04-25
Resource type
Major
Rights statement

Relations

In Collection:

Items

Items

Permanent link to this page: https://digital.wpi.edu/show/6t053h404