Faculty Advisor

Vidali, Luis

Center

UMMC / University of Massachusetts Medical Center

Abstract

In the plant model system, the moss Physcomitrella patens, the motor protein myosin XI is essential for polarized cell growth and proliferation. Myosin XI is structurally similar to Myosin V in animals. Myosin V was found to interact, via its globular tail domain, with the exocyst subunit sec15 during secretory vesicle transport in yeast and therefore we hypothesize myosin XI and sec15 would interact in P. patens. In this study, we recombinantly expressed and purified two globular tail domain constructs from myosin XI and full length sec15 from P. patens. The purified proteins can be used in future experiments to analyze the proposed binding interaction of myosin XI and sec15.

Publisher

Worcester Polytechnic Institute

Date Accepted

April 2013

Major

Biology and Biotechnology

Project Type

Major Qualifying Project

Accessibility

Unrestricted

Advisor Department

Biology and Biotechnology

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