Faculty Advisor

DiBiasio, David


In this report, a more efficient technique to produce mAbs from Chinese Hamster Ovary cells in fed-batch systems is presented. A feed-forward control mechanism was created with BioXpert. The results are not conclusive due to lack of replicates to demonstrate reproducibility. If they are correct, it may be concluded that feed-forward control system was successfully implemented on the fed-batch bioreactors via BioXpert. Titer results from BioXpert experiments yielded higher results than manual feed-forward and similar to previous feedback experiments. For future work, it is recommended to repeat BioXpert feed-forward experiment with two or more replicates maintaining same cell line and using more frequent feeding schedule by keeping total daily feeding amount the same.


Worcester Polytechnic Institute

Date Accepted

April 2008


Chemical Engineering

Project Type

Major Qualifying Project



Advisor Department

Chemical Engineering

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