University of Massachusetts Medical School
Inflammation is initiated by the innate immune system to remove harmful stimuli and heal infections. Multiprotein complexes, called inflammasomes, process inflammatory cytokines such as interleukin (IL)-1 beta and IL-18 and are essential for optimal host response. Infammasomes are typically comprised of one or more nod-like receptor (NLR), a pro-caspase protease, and an adaptor molecule. NLRP3 and NLRP12 are NLRs with specificities towards certain pathogens and vaccines; however, aspects of their activation remain unclear. To investigate a possible mode of assembly, we generated fluorescently tagged NLR pyrin domain-containing protein (NLRP) plasmids for transformation into cells. Expression of NLRP3 and NLRP12 inflammasome components was successfully observed by fluorescence microscopy.
Worcester Polytechnic Institute
Biology and Biotechnology
Major Qualifying Project
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Chemistry and Biochemistry