Faculty Advisor

Adams, David S.

Abstract

Influenza is a major cause of human illness and death, and as such requires vast attention.Current vaccine research shows that human sera have influenza virus-specific antibodies which mediate the complement-dependent lysis(CDL) of viral infected cells and these antibodies may be more cross-reactive than typical neutralizing antibodies.This project focused on the development of a protein expression system that would allow human A549 cells to express influenza proteins to be used in analyzing the specificity of CDL antibodies.The results show that a lentiviral expression system is not the best method of expression; only H3 protein expression was confirmed by immunoblotting. Several influenza gene constructs were then successfully constructed for future vaccinia virus expression experiments.

Publisher

Worcester Polytechnic Institute

Date Accepted

January 2011

Major

Biology and Biotechnology

Project Type

Major Qualifying Project

Accessibility

Unrestricted

Advisor Department

Biology and Biotechnology

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