Faculty Advisor

Gericke, Arne

Abstract

IQGAP1 is a ubiquitously expressed protein that is a member of the IQGAP family of scaffolding proteins. IQGAP1 has been found to regulate a diverse range of cellular processes including: cell migration, cell proliferation, cytoskeletal dynamics, and intracellular signaling. Previous studies indicated that IQGAP1 binds to phosphoinositides. Interactions between IQGAP1 and phosphoinositides can be characterized by in-vitro studies using purified proteins and phosphoinositide containing model membranes. Because IQGAP1 is a large multi-domain protein, purifying it presented challenges. Therefore, IQGAP1-C, a truncated protein that includes the proposed PIP binding site, was instead purified. This study shows that we were able to purify IQGAP1-C and characterize its interaction with lipids.

Publisher

Worcester Polytechnic Institute

Date Accepted

April 2018

Major

Biochemistry

Project Type

Major Qualifying Project

Accessibility

Unrestricted

Advisor Department

Chemistry and Biochemistry

Advisor Program

Chemistry and Biochemistry

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