IQGAP1 is a ubiquitously expressed protein that is a member of the IQGAP family of scaffolding proteins. IQGAP1 has been found to regulate a diverse range of cellular processes including: cell migration, cell proliferation, cytoskeletal dynamics, and intracellular signaling. Previous studies indicated that IQGAP1 binds to phosphoinositides. Interactions between IQGAP1 and phosphoinositides can be characterized by in-vitro studies using purified proteins and phosphoinositide containing model membranes. Because IQGAP1 is a large multi-domain protein, purifying it presented challenges. Therefore, IQGAP1-C, a truncated protein that includes the proposed PIP binding site, was instead purified. This study shows that we were able to purify IQGAP1-C and characterize its interaction with lipids.
Worcester Polytechnic Institute
Major Qualifying Project
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Chemistry and Biochemistry