Faculty Advisor

Dominko, Tanja

Abstract

It has been found that hypoxia is an advantageous condition for the culture of pluripotent stem cells. Hypoxia-inducible factors (HIFs), are transcription factors involved in the mediation of a hypoxic response. HIF has downstream gene targets that have implications in pluripotency. The current research attempts to characterize their activity in human adult fibroblasts. Specifically, it aims to determine if HIFs are responsive to hypoxia in fibroblasts and whether their regulation is affected at transcriptional, translational or post-translational levels. Further, HIF localization was examined as an implication of protein function. It was found that HIFs are expressed in fibroblasts, but the hypoxic response is different than observed in human embryonic stem cells.

Publisher

Worcester Polytechnic Institute

Date Accepted

April 2011

Major

Biology and Biotechnology

Major

Psychological Science

Project Type

Major Qualifying Project

Accessibility

Unrestricted

Advisor Department

Biology and Biotechnology

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