Faculty Advisor

Vidali, Luis

Abstract

It has been hypothesized that myosin and other cell components are located on vesicles to organize the actin network during polarized growth. Using confocal microscopy and fluctuation cross correlation analyses, I investigated the association of class II formins with vesicles and F-actin in Physcomitrella patens caulonemal cells. I found that formin2A co-localized with both VAMP, a vesicle marker, and Lifeact, an F-actin probe. Furthermore, formin2A fluctuated in phase with VAMP, but preceded Lifeact, in agreement with the hypothesis.

Publisher

Worcester Polytechnic Institute

Date Accepted

April 2015

Major

Biology and Biotechnology

Project Type

Major Qualifying Project

Accessibility

Unrestricted

Advisor Department

Biology and Biotechnology

Share

COinS