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A Liquid Assay for Screening Fungal Virulence Factors & kgb-1;fer-1 Double Mutant Construction

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It is difficult to develop drugs against fungal infections because both fungi and their hosts are eukaryotic. By using a host-pathogen system and an assay in high-throughput screens of genetic knockouts, fungal virulence factors and host immunity modulators can be identified and studied. We are developing a liquid culture assay to study the effects of co-culturing C. elegans with S. cerevisiae mutants. We tested several conditions including different liquid media, E.coli to Yeast mass ratios, and C. elegans mutant strains. We concluded that there are other factors causing the death of the C. elegans in the assay, such as matricide, which are not S. cerevisiae-related. To exclude matricide, a new C. elegans strain with kgb-1; fer-1 double mutant genotype was constructed by crossing.

  • This report represents the work of one or more WPI undergraduate students submitted to the faculty as evidence of completion of a degree requirement. WPI routinely publishes these reports on its website without editorial or peer review.
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Identifier
  • E-project-010909-214819
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Year
  • 2009
Date created
  • 2009-01-09
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Last modified
  • 2021-02-02

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