Faculty Advisor

Dominko, Tanja

Faculty Advisor

Rolle, Marsha W.

Center

OTHER / Other location, not listed

Abstract

It is difficult to predict the toxic effects of pesticides on humans because traditional 2D cell culture does not provide physiologically relevant data. As a result, we designed a process utilizing 3D cell culture as a model to study the cytotoxicity caused by pesticide exposure. Mn9D cells are well-established as a model of studying neurodegeneration and have not been published in 3D cell culture. Thus, we developed a new approach to form 3D spheroids, with Mn9D cells to model the cellular interactions of neurons. We tested various cell suspension platforms and spheroid characteristics to determine the optimal spheroid. We found that PDMS-coated 96 well plates with 5,000 cells per well formed spheroids with the highest degree of compactness, with an average sphericity of .958 ± .030.

Publisher

Worcester Polytechnic Institute

Date Accepted

January 2014

Major

Biomedical Engineering

Project Type

Major Qualifying Project

Accessibility

Unrestricted

Advisor Department

Biology and Biotechnology

Advisor Department

Biomedical Engineering

Share

COinS