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Perturbation Analysis of a Potassium Channel Beta Subunit

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KCNQ1 is a voltage-gated potassium channel that co-assembles with the KCNE family of transmembrane peptides. KCNQ1/KCNE3 channels are found in colonic crypt and airway epithelial cells and are constitutively conducting at normal resting potentials. Using site-directed mutagenesis, residues 87-96 in the C-terminus were mutated to alanine. The RNA of these mutants was microinjected into Xenopus oocytes and examined using a two-electrode voltage clamp. Results were compared to KCNE1 to determine the role of C-terminus in KCNQ1 modulation.

  • This report represents the work of one or more WPI undergraduate students submitted to the faculty as evidence of completion of a degree requirement. WPI routinely publishes these reports on its website without editorial or peer review.
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Identifier
  • E-project-042606-192358
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Year
  • 2006
Date created
  • 2006-04-26
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Major
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Last modified
  • 2020-12-27

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