Faculty Advisor

Wobbe, Kristin K

Abstract

KCNQ1 is a voltage-gated potassium channel that co-assembles with the KCNE family of transmembrane peptides. KCNQ1/KCNE3 channels are found in colonic crypt and airway epithelial cells and are constitutively conducting at normal resting potentials. Using site-directed mutagenesis, residues 87-96 in the C-terminus were mutated to alanine. The RNA of these mutants was microinjected into Xenopus oocytes and examined using a two-electrode voltage clamp. Results were compared to KCNE1 to determine the role of C-terminus in KCNQ1 modulation.

Publisher

Worcester Polytechnic Institute

Date Accepted

April 2006

Major

Biochemistry

Project Type

Major Qualifying Project

Accessibility

Unrestricted

Advisor Department

Chemistry and Biochemistry

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