Wobbe, Kristin K
KCNQ1 is a voltage-gated potassium channel that co-assembles with the KCNE family of transmembrane peptides. KCNQ1/KCNE3 channels are found in colonic crypt and airway epithelial cells and are constitutively conducting at normal resting potentials. Using site-directed mutagenesis, residues 87-96 in the C-terminus were mutated to alanine. The RNA of these mutants was microinjected into Xenopus oocytes and examined using a two-electrode voltage clamp. Results were compared to KCNE1 to determine the role of C-terminus in KCNQ1 modulation.
Worcester Polytechnic Institute
Major Qualifying Project
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Chemistry and Biochemistry