Faculty Advisor

Adams, David S.

Faculty Advisor

Page, Raymond L.

Abstract

Many current systems for the development of myofibers involve the presence of a scaffold, which can be a physical barrier to cell proliferation and cause incongruities in the myofiber. We developed a molding system into which myogenic cells can be seeded to form a myofiber without the need for a scaffold. A "dog-bone" shaped molding system with a seeding channel and incorporated anchor posts was found to result in tissue formation one day after seeding. Characterization of C2C12 cells showed that differentiation occurs at Day 4 post-induction of differentiation and myosin staining of C2C12 cells confirmed maturation of resulting myofibers. Further studies should focus on improved manufacture of the mold to enhance the long-term stability of the myofiber.

Publisher

Worcester Polytechnic Institute

Date Accepted

April 2011

Major

Biology and Biotechnology

Major

Biomedical Engineering

Project Type

Major Qualifying Project

Accessibility

Unrestricted

Advisor Department

Biology and Biotechnology

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