Faculty Advisor

Buckholt, Michael Allan

Center

University of Massachusetts Medical Center

Abstract

Mdm2 and MdmX are homologous proteins that function as oncogenes by negatively regulating the tumor suppressor p53. The overexpression of each has been linked to the tumorigenesis and metastasis of a significant portion of human cancers. Recent studies have shown that MdmX can also function as a tumor suppressor by promoting genome stability in a p53-independent manner. Data has now further identified that the Zinc Finger (ZnF) region of MdmX is the key determinant to this function. This project aimed to generate, validate, and functionally analyze mouse epithelial tumor cell lines suitable for the future identification of MdmX interactive protein partners. Also of interest was to explore whether the role of MdmX in supporting genome stability requires intact Mdm2 function.

Publisher

Worcester Polytechnic Institute

Date Accepted

February 2016

Major

Biology and Biotechnology

Project Type

Major Qualifying Project

Accessibility

Unrestricted

Advisor Department

Biology and Biotechnology

Project Center

University of Massachusetts Medical Center

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