Faculty Advisor

Ryder, Elizabeth F

Abstract

Membrane trafficking is a major cellular mechanism for the regulation of surface expression of plasma membrane proteins. Recently, a carboxyl terminus motif, FREKLAYAIA, has been shown to control the internalization of the dopamine transporter (DAT), a critical protein in synaptic transmission. Sequence comparison across BLAST identified a similar carboxyl motif, SREKLQYSIP, in the leak potassium channel KCNK3. The current study tested whether this motif was necessary and sufficient for the internalization of the channel. Our results suggest that the carboxyl terminus of KCNK3 is sufficient for the internalization of an endocytic deficient reporter molecule (Tac), and that the SREKLQYSIP motif may be necessary for this internalization.

Publisher

Worcester Polytechnic Institute

Date Accepted

April 2007

Major

Biology and Biotechnology

Major

Biochemistry

Project Type

Major Qualifying Project

Accessibility

Unrestricted

Advisor Department

Biology and Biotechnology

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