Faculty Advisor

Adams, David S.

Abstract

Mesenchymal stem cells (MSCs) are multipotent stromal cells that have the potential to differentiate into various cells lineages, including adipocytes, chondrocytes, osteocytes, and fibroblasts. Due to their ability to regenerate damaged tissues, MSCs have the potential to be the future of new cell-based therapies. However, identification of MSCs has not been standardized. The purpose of this project was to identify parameters for isolating MSCs from murine bone marrow. This was accomplished by combining a staining protocol that utilizes the unique surface characteristics of MSCs with the negative selection for CD45 and Ter-119, and then the negative selection for CD44 in the remaining cell population.

Publisher

Worcester Polytechnic Institute

Date Accepted

April 2013

Major

Biology and Biotechnology

Project Type

Major Qualifying Project

Accessibility

Unrestricted

Advisor Department

Biology and Biotechnology

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