Faculty Advisor

Adams, David S.

Center

University of Massachusetts Medical Center

Abstract

Activated CD4+ T-cells targeting specific auto-antigenic peptides of myelin proteins mediate the pathogenesis in multiple sclerosis patients and EAE models. The disease mechanisms that cause MS remain unknown, but susceptibility maps to specific HLA-DR beta- 1 alleles (especially 04.01, and 15.01). To investigate the genetic predisposition of MS recombinant HLA-DR beta-1 complexes (01.01, 04.01, and 15.01) were constructed containing different peptides derived from sheath proteins: PLP, MBP, and MOG. The gene constructs were transfected into SF9 cells to evaluate surface expression as it relates to the auto-antigenic activity of several pMHC combinations. Individual myelin peptides were also assayed for the ability to induce EAE in transgenic HLA-DR beta-1 04.01 and 15.01 mice.

Publisher

Worcester Polytechnic Institute

Date Accepted

April 2015

Major

Biology and Biotechnology

Project Type

Major Qualifying Project

Accessibility

Unrestricted

Advisor Department

Biology and Biotechnology

Project Center

University of Massachusetts Medical Center

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