Faculty Advisor

Adams, David S.

Center

UMMC / University of Massachusetts Medical Center

Abstract

Activated CD4+ T-cells targeting specific auto-antigenic peptides of myelin proteins mediate the pathogenesis in multiple sclerosis patients and EAE models. The disease mechanisms that cause MS remain unknown, but susceptibility maps to specific HLA-DR beta- 1 alleles (especially 04.01, and 15.01). To investigate the genetic predisposition of MS recombinant HLA-DR beta-1 complexes (01.01, 04.01, and 15.01) were constructed containing different peptides derived from sheath proteins: PLP, MBP, and MOG. The gene constructs were transfected into SF9 cells to evaluate surface expression as it relates to the auto-antigenic activity of several pMHC combinations. Individual myelin peptides were also assayed for the ability to induce EAE in transgenic HLA-DR beta-1 04.01 and 15.01 mice.

Publisher

Worcester Polytechnic Institute

Date Accepted

April 2015

Major

Biology and Biotechnology

Project Type

Major Qualifying Project

Accessibility

Unrestricted

Advisor Department

Biology and Biotechnology

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