Microbial infections of gram-negative bacteria in Drosophila are recognized through the immune deficiency (IMD) pathway by a molecular mechanism not completely understood. This project initiated an analysis of the potential IMD role of PGRP-LE, a peptidoglycan recognition protein that binds bacterial cell wall fragments to activate intracellular signaling. Four PGRP-Le mutants were created (E231L, S232E, R254T, and a S232E/R254T double mutant) using genomic rescue transgenes cloned into a pattB vector. A MDP transporter, Yin, was tested as a potential intracellular transporter for TCT molecules, but found as improbable. This research will allow further study of the molecular mechanism of the IKK-mediated Relish activation in IMD pathways.

• This report represents the work of one or more WPI undergraduate students submitted to the faculty as evidence of completion of a degree requirement. WPI routinely publishes these reports on its website without editorial or peer review.

Creator

• Rich, Scott Agersea

Publisher

• Worcester Polytechnic Institute

Identifier

• E-project-042612-000548

Advisor

• Adams, David S.

Year

• 2012

Sponsor

• University of Massachusetts Medical Center.
• Neal Silverman

Date created

• 2012-04-26

Location

• Worcester

Resource type

• Major Qualifying Project

Major

• Biology & Biotechnology

Rights statement

• In Copyright