Faculty Advisor

Adams, David S.

Project Center

University of Massachusetts Medical Center


Microbial infections of gram-negative bacteria in Drosophila are recognized through the immune deficiency (IMD) pathway by a molecular mechanism not completely understood. This project initiated an analysis of the potential IMD role of PGRP-LE, a peptidoglycan recognition protein that binds bacterial cell wall fragments to activate intracellular signaling. Four PGRP-Le mutants were created (E231L, S232E, R254T, and a S232E/R254T double mutant) using genomic rescue transgenes cloned into a pattB vector. A MDP transporter, Yin, was tested as a potential intracellular transporter for TCT molecules, but found as improbable. This research will allow further study of the molecular mechanism of the IKK-mediated Relish activation in IMD pathways.


Worcester Polytechnic Institute

Date Accepted

April 2012


Biology and Biotechnology

Project Type

Major Qualifying Project



Advisor Department

Biology and Biotechnology

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