Faculty Advisor

Adams, David S.

Center

University of Massachusetts Medical Center

Abstract

Microbial infections of gram-negative bacteria in Drosophila are recognized through the immune deficiency (IMD) pathway by a molecular mechanism not completely understood. This project initiated an analysis of the potential IMD role of PGRP-LE, a peptidoglycan recognition protein that binds bacterial cell wall fragments to activate intracellular signaling. Four PGRP-Le mutants were created (E231L, S232E, R254T, and a S232E/R254T double mutant) using genomic rescue transgenes cloned into a pattB vector. A MDP transporter, Yin, was tested as a potential intracellular transporter for TCT molecules, but found as improbable. This research will allow further study of the molecular mechanism of the IKK-mediated Relish activation in IMD pathways.

Publisher

Worcester Polytechnic Institute

Date Accepted

April 2012

Major

Biology and Biotechnology

Project Type

Major Qualifying Project

Accessibility

Unrestricted

Advisor Department

Biology and Biotechnology

Project Center

University of Massachusetts Medical Center

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