Faculty Advisor

Adams, David S.

Abstract

MicroRNAs (miRNAs) are non-coding double stranded RNA molecules known to regulate gene expression. The recent discovery that the miR-30 family of miRNAs overexpress in mouse breast cancer tumor cells in the same regions commonly seen in human breast cancer tumor cells, has lead us to design a system for over-expressing these miRNAs to help evaluate their role in tumorigenesis. In this study, genes encoding miR-30c1, c2, a, and d, were successfully cloned. The gene for miR-30c1 was further investigated in a tet inducible expression system. Fluorescence data indicated HEK-293 cells were successfully transfected with expression plasmid, and qRT-PCR on HEK-293 RNA indicated miR-30c1 levels increased.

Publisher

Worcester Polytechnic Institute

Date Accepted

May 2009

Major

Biology and Biotechnology

Project Type

Major Qualifying Project

Accessibility

Unrestricted

Advisor Department

Biology and Biotechnology

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