Faculty Advisor
Adams, David S.
Sponsor
UMass Medical School
Abstract
MicroRNAs (miRNAs) are non-coding double stranded RNA molecules known to regulate gene expression. The recent discovery that the miR-30 family of miRNAs overexpress in mouse breast cancer tumor cells in the same regions commonly seen in human breast cancer tumor cells, has lead us to design a system for over-expressing these miRNAs to help evaluate their role in tumorigenesis. In this study, genes encoding miR-30c1, c2, a, and d, were successfully cloned. The gene for miR-30c1 was further investigated in a tet inducible expression system. Fluorescence data indicated HEK-293 cells were successfully transfected with expression plasmid, and qRT-PCR on HEK-293 RNA indicated miR-30c1 levels increased.
Publisher
Worcester Polytechnic Institute
Date Accepted
May 2009
Major
Biology and Biotechnology
Project Type
Major Qualifying Project
Copyright Statement
All authors have granted to WPI a nonexclusive royalty-free license to distribute copies of the work, subject to other agreements. Copyright is held by the author or authors, with all rights reserved, unless otherwise noted.
Accessibility
Unrestricted
Advisor Department
Biology and Biotechnology