University of Massachusetts Medical Center
Modularly assembled Zinc Finger Proteins (ZFPs) have been created to recognize a majority of three base pair target sites, yet, gaps exist in their ability to recognize 5’-TNN-3’ target sites. Utilizing the Homeodomain’s ability to bind thiamine and adenine rich sequences in combination with ZFPs, these recognition gaps can be resolved. Here, the composition of an inter-domain linker between a Homeodomain and a two finger ZFP was optimized to create chimeras that bind with high affinity and specificity.
Worcester Polytechnic Institute
Biology and Biotechnology
Major Qualifying Project
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Chemistry and Biochemistry
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