Faculty Advisor

Heilman, Destin

Center

University of Massachusetts Medical Center

Abstract

Modularly assembled Zinc Finger Proteins (ZFPs) have been created to recognize a majority of three base pair target sites, yet, gaps exist in their ability to recognize 5’-TNN-3’ target sites. Utilizing the Homeodomain’s ability to bind thiamine and adenine rich sequences in combination with ZFPs, these recognition gaps can be resolved. Here, the composition of an inter-domain linker between a Homeodomain and a two finger ZFP was optimized to create chimeras that bind with high affinity and specificity.

Publisher

Worcester Polytechnic Institute

Date Accepted

April 2013

Major

Biology and Biotechnology

Project Type

Major Qualifying Project

Accessibility

Unrestricted

Advisor Department

Chemistry and Biochemistry

Project Center

University of Massachusetts Medical Center

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