Monoclonal antibodies are proven to be an extremely successful class of protein therapeutics. Monoclonal antibodies and other biopharmaceutical products used in humans, are required to meet strict purity standards. Cation-exchange (CIEx) chromatography has become a staple of monoclonal antibody purification platforms. In this project, an experimental chromatography resin specifically designed to be operated in flow-through mode and replace traditional CIEx during monoclonal antibody polishing has been characterized. The results show that this new resin can achieve higher loading capacities and reduced buffer consumption, resulting in improved process economics, while being an effective replacement for currently available CIEx resins that are designed to be operated in bind-and-elute mode.

• This report represents the work of one or more WPI undergraduate students submitted to the faculty as evidence of completion of a degree requirement. WPI routinely publishes these reports on its website without editorial or peer review.

Creator

• Czapkowski, Brooke Leslie

Publisher

• Worcester Polytechnic Institute

Identifier

• E-project-042413-101048

Advisor

• Adams, David S.

Year

• 2013

Sponsor

• EMD Millipore

Date created

• 2013-04-24

Resource type

• Major Qualifying Project

Major

• Biochemistry

Rights statement

• In Copyright