Faculty Advisor

Adams, David S.

Abstract

Monoclonal antibodies are proven to be an extremely successful class of protein therapeutics. Monoclonal antibodies and other biopharmaceutical products used in humans, are required to meet strict purity standards. Cation-exchange (CIEx) chromatography has become a staple of monoclonal antibody purification platforms. In this project, an experimental chromatography resin specifically designed to be operated in flow-through mode and replace traditional CIEx during monoclonal antibody polishing has been characterized. The results show that this new resin can achieve higher loading capacities and reduced buffer consumption, resulting in improved process economics, while being an effective replacement for currently available CIEx resins that are designed to be operated in bind-and-elute mode.

Publisher

Worcester Polytechnic Institute

Date Accepted

April 2013

Major

Biochemistry

Project Type

Major Qualifying Project

Accessibility

Unrestricted

Advisor Department

Biology and Biotechnology

Share

COinS