Adams, David S.
Monoclonal antibodies are proven to be an extremely successful class of protein therapeutics. Monoclonal antibodies and other biopharmaceutical products used in humans, are required to meet strict purity standards. Cation-exchange (CIEx) chromatography has become a staple of monoclonal antibody purification platforms. In this project, an experimental chromatography resin specifically designed to be operated in flow-through mode and replace traditional CIEx during monoclonal antibody polishing has been characterized. The results show that this new resin can achieve higher loading capacities and reduced buffer consumption, resulting in improved process economics, while being an effective replacement for currently available CIEx resins that are designed to be operated in bind-and-elute mode.
Worcester Polytechnic Institute
Major Qualifying Project
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Biology and Biotechnology
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