Faculty Advisor

Adams, David S.

Abstract

Adult enhancer factor-1 (Aef-1) is a 4 finger DNA-binding zinc finger protein which functions as an adult alcohol dehydrogenase gene (Adh) repressor in Drosophila. During larvae development it also plays a role in decreasing expression of the Adh gene. This project used mutagenesis of the Aef-1 gene and the bacterial one-hybrid system to examine DNA binding specificity and recognition of the Aef-1 zinc fingers. The intent of this project was to investigate the importance of each zinc finger, and binding specificity of a 3 finger Aef-1 construct.

Publisher

Worcester Polytechnic Institute

Date Accepted

April 2007

Major

Biology and Biotechnology

Project Type

Major Qualifying Project

Accessibility

Unrestricted

Advisor Department

Biology and Biotechnology

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