Cloning and Expression of H2-D(b) for Future Experimental Studies on its Role in Multiple Sclerosis
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open in viewerMultiple sclerosis is an inflammatory disease characterized by damage to neuronal fatty myelin sheaths and neuronal dysfunction. Studies have shown that tg mice expressing a T cell receptor reactive to GFAP can succumb to spontaneous autoimmunity with pathologies comparable to MS. This study focused on the expression of a MHC-I type H2-D(b) Beta-2m receptor domain in pBac & cloning H2-D(b) into pLM-1 expression vector. The Beta-2m domain expression vector was transformed into BL21 cells, expression was confirmed via SDS-PAGE. The H2-D(b) genes were then successfully cloned into the pLM-1 expression vector. Expression of Beta-2m receptor domain and of H2-Db/GFAP ligand complex will be used for further experimentation to observe abnormalities within the H2-D(b) epitope and its role in MS.
- This report represents the work of one or more WPI undergraduate students submitted to the faculty as evidence of completion of a degree requirement. WPI routinely publishes these reports on its website without editorial or peer review.
- Creator
- Publisher
- Identifier
- E-project-042513-160359
- Advisor
- Year
- 2013
- Sponsor
- Date created
- 2013-04-25
- Resource type
- Major
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