Faculty Advisor

Adams, David S.

Abstract

Murine polyomavirus, a double stranded DNA virus that causes multiple kinds of tumors, was one of the first viruses ever sequenced, and has proven to be an invaluable model in virology, cancer research, molecular biology and immunology. The purpose of this MQP was to use FACS staining to isolate viral antigen (VP1 major capsid protein)-specific B-lymphocytes using a double stain for VP1 and B220. A major portion of the project focused on designing a treatment to overcome the non-specific binding of antibodies to cell surface sialic acid residues. The enrichment of antigen-specific B cells succeeded, and these cells can be analyzed for gene expression patterns to further understand these virus-specific cells of the humoral immunity.

Publisher

Worcester Polytechnic Institute

Date Accepted

April 2006

Major

Biology and Biotechnology

Project Type

Major Qualifying Project

Accessibility

Unrestricted

Advisor Department

Biology and Biotechnology

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