Faculty Advisor

Dominko, Tanja

Faculty Advisor

Page, Raymond L.

Abstract

Muscle injury therapy employs human primary satellite skeletal muscle cells seeded onto micro-threads. However, the cell culture process prior to seeding that would assure maintenance of the myogenic potential has not been optimized. This cell line has both adherent and non-adherent cell populations; non-adherent population displaying higher myogenic potential. The concentrations of selected components of the cell culture medium were varied to determine their effect on the proportion of non-adherent satellite cells. The subsequent effect on myogenic potential was determined by inducing the cells to differentiate by culture in differentiation medium and calculating the myogenic index. An economic analysis was performed to determine the cost-benefit of implementing the optimized process.

Publisher

Worcester Polytechnic Institute

Date Accepted

April 2013

Major

Biology and Biotechnology

Major

Biomedical Engineering

Project Type

Major Qualifying Project

Accessibility

Unrestricted

Advisor Department

Biology and Biotechnology

Advisor Department

Biomedical Engineering

Share

COinS