Faculty Advisor

Flaherty, Patrick J.

Abstract

Advances in error correction for next generation sequencing have not matched increases in data production and as a result, the quality of the generated nucleotide sequences has suffered. The purpose of this project was to develop a processing pipeline which would remove errors from intensity data for a faster and more accurate analysis. The method employed to achieve these goals was to redesign the algorithm used to correct for bleaching and phasing to capture a greater number of misidentified bases. Two pipelines were created, pipeline 1 (illumina) and pipeline 2 (Oracle ), it was determined that pipeline 2 out preformed pipeline one in terms of accuracy. But pipeline 1 was determined to be faster in processing time and thus the main question is asked do you sacrifice time for efficiency?

Publisher

Worcester Polytechnic Institute

Date Accepted

April 2014

Major

Biomedical Engineering

Major

Humanities and Arts

Project Type

Major Qualifying Project

Accessibility

Unrestricted

Advisor Department

Biomedical Engineering

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