Myosins, a superfamily of actin dependent molecular motors, perform essential functions in all eukaryotes. Full length functional plant myosins have been difficult to isolate due to their sus-ceptibility to proteases. Using Western blots for detection, the degradation of 3xmEGFP-tagged myosin XI was compared for different methods of extraction from the moss Physcomitrella pat-ens. Motility assays showed the functionality of moss myosin enriched by an actin based affinity column. This work begins the development of an effective and reproducible purification protocol for a moss class XI myosin. Analysis of quantitative assays and biochemical studies of purified myosin will allow the characterization of the physiological roles underlying the evolution and function of this essential protein.
Worcester Polytechnic Institute
Major Qualifying Project
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Biology and Biotechnology