Faculty Advisor

Buckholt, Michael Allan

Faculty Advisor

Srinivasan, Jagan

Abstract

Nematodes can be found in a variety of habitats and environmental conditions. Knowing what types of nematodes can be found in an area can help assess environmental health. This project, examines the diversity of nematodes in the Central Massachusetts region, where no nematode biodiversity studies have been done before. Nematodes were isolated from 17 towns within the region. Abundance of nematodes and isolation time varied between samples. Results show that there is an optimal pH range for nematode isolation falling within 4.51-5.00. PCR was completed to amplify the worm DNA using the SSU genetic marker. Sequencing was then done on amplified DNA samples from Hopedale. Two Pristionchus species were found: P. marianneae and P. pauli.

Publisher

Worcester Polytechnic Institute

Date Accepted

July 2013

Major

Biology and Biotechnology

Project Type

Major Qualifying Project

Accessibility

Unrestricted

Advisor Department

Biology and Biotechnology

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